BioBits - The Central Dogma
The Micropipette Challenge is an activity developed to introduce students to a commonly used laboratory tool called the micropipette.
A micropipette is often used for measuring, transferring, or injecting very small quantities of liquid and can be used across all fields of science, including, chemistry, biology, forensic science, pharmaceutics, etc. Micropipettes use a scale of measurement that is based on the metric system. The metric system is a system of measurement based on the powers of 10 and is also synonymous with the System of International Units, which is the most widely used system of measurement.
In the Micropipette Challenge lesson, students work with small amounts of liquid to create a visible spectrum. This is a great introductory lesson that allows students to gain experience with the tools and techniques used in other SPARC learning labs. At the end of the module, they will use their knowledge an experience to create a design of their choosing. Importantly, students will also learn how to carefully read and follow a scientific protocol and will gain experience working with units in the metric system.
Upon completion of the Micropipette Challenge Lab, students will be able to:
* Understand how to properly use a micropipette
* Read and follow a laboratory protocol
* Perform unit conversions, with an emphasis on microliters to milliliters
Students should have a basic understanding of a micropipette (what is it?). Don't worry, we will go over how to use it before jumping into the activity. Students should also have a general understanding of the metric system and the common metric units used for volumes. A review of the metric system and metric conversions can be found here:
Math is Fun
Roy, Gee, and Biv were asked by their teacher to create a spectrum, but they have no idea what to do and need your help! Using red, blue, and yellow solutions, you will make a visible spectrum, but in order to correctly do this, you will need to use a micropipette. Employing your knowledge of the metric system and unit conversions, you will learn how to operate a micropipette and measure small quantities of liquids to create your spectrum.
Specifications and Requirements
Skill Level: Basic
Focus: Laboratory tools, Laboratory skills, Metric system, Following a protocol
Time: 45 minutes
Physical Space: Enough room for groups of 3-4 students to interact together and perform the laboratory activity.
Students should be able to answer these questions before beginning the Micropipette Challenge. Also, refer to the Pre-Lab Activity above.
* What is the metric system?
* What is the SI system of measurement?
* What countries use the metric system? What system of measurement does the
United States use?
* What is a unit conversion? What is a conversion factor?
* How do you convert from milliliters to liters?
* Why do you think it is important that scientists use units in their work?
What professions use micropipettes? Check them out!
Biochemists and Biophysicists study the chemical and physical principles of living things and of biological processes, such as cell development, growth, heredity, and disease. Learn more: https://www.bls.gov/ooh/life-physical-and-social-science/biochemists-and-biophysicists.htm
Biomedical Engineers combine engineering principles with medical sciences to design and create equipment, devices, computer systems, and software used in healthcare. Learn more: https://www.bls.gov/ooh/architecture-and-engineering/biomedical-engineers.htm
Chemists/Material Scientists study substances at the atomic and molecular levels and analyze the ways in which the substances interact with one another. They use their knowledge to develop new and improved products and to test the quality of manufactured goods. Learn more: https://www.bls.gov/ooh/life-physical-and-social-science/chemists-and-materials-scientists.htm
Epidemiologists are public health professionals who investigate patterns and causes of disease and injury in humans. They seek to reduce the risk and occurrence of negative health outcomes through research, community education, and health policy. Learn more: https://www.bls.gov/ooh/life-physical-and-social-science/epidemiologists.htm
Medical Scientists conduct research aimed at improving overall human health. They often use clinical trials and other investigative methods to reach their findings. Learn more: https://www.bls.gov/ooh/life-physical-and-social-science/medical-scientists.htm
Biological Technicians help biological and medical scientists conduct laboratory tests and experiments. Learn more: https://www.bls.gov/ooh/life-physical-and-social-science/biological-technicians.htm
Microbiologists study microorganisms such as bacteria, viruses, algae, fungi, and some types of parasites. They try to understand how these organisms live, grow, and interact with their environments. Learn more: https://www.bls.gov/ooh/life-physical-and-social-science/microbiologists.htm
Zoologists and Wildlife Biologists study animals and other wildlife and how they interact with their ecosystems. They study the physical characteristics of animals, animal behaviors, and the impacts humans have on wildlife and natural habitats. Learn more: https://www.bls.gov/ooh/life-physical-and-social-science/zoologists-and-wildlife-biologists.htm